Bio-Rad CD54抗体 AHP2183B,Rabbit anti Human CD54:Biotin-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad CD54抗体 AHP2183B,Rabbit anti Human CD54:Biotin

2024-10-22

Rabbit anti Human CD54:Biotin

货号：AHP2183B

规格：50 µg

价格：4208

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：CD54

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Biotin

产品介绍
Rabbit anti Human CD54 antibodyrecognizes human CD54, otherwise known as Intercellular adhesion molecule 1 or ICAM-1. CD54 is a 532 amino acid single pass type I membrane glycoprotein containing fiveIg-like C2-type domains. CD54 is widely expressed and acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, it is the major receptor for human rhinovirus(Stauntonet al.1989).CD54 is highly expressed on activated endothelial cells (Favoloro 1993) and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress (Manes and Pober 2011). Levels of soluble CD54 in serum (Matalliotakiset al.2001) or urine (Kimet al.2006), can be potentially used as an indication of infection, inflammation and certain tumour, and as a target for immunosuppression in transplantation.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Biotin - lyophilized
Reconstitution	Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
AntiserumPreparation	Antiserum to human CD54 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Carrier Free	Yes
Immunogen	Recombinant human CD54.
Approx. Protein Concentrations	IgG concentration 0.1mg/ml after reconstitution
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	0.25	1.0ug/ml
Western Blotting	√	0.1	0.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. ELISA This biotinylated human CD54 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with ourpurified human CD54 antibody(AHP2183) as the capture reagent andrecombinant human CD54(PHP250) as the standard.
文献
1.Nadlonek, N.A.et al.(2012) Radiation induces osteogenesis in human aortic valve interstitial cells.J Thorac Cardiovasc Surg. 144 (6): 1466-70.2.Watson, R.L.et al.(2015) Endothelial CD99 signals through soluble adenylyl cyclase and PKA to regulate leukocyte transendothelial migration.J Exp Med. 212 (7): 1021-41.

技术参数

产品应用 E ;WB

产品类型 Polyclonal Antibody

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Bio-Rad CD54抗体 AHP2183B,Rabbit anti Human CD54:Biotin

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