Bio-Rad AHP1891 ATG12 (Internal Region),Rabbit anti ATG12 (Internal Region) antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1891 ATG12 (Internal Region),Rabbit anti ATG12 (Internal Region) antibody

2024-10-22

Rabbit anti ATG12 (Internal Region) antibody

货号：AHP1891

规格：0.1 mg

价格：4692

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：ATG12 (Internal Region)

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 488

产品介绍
Rabbit anti ATG12 (Internal Region) antibodyrecognizes human Autophagy-related protein 12 (ATG12).ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.There are at least two isoforms of ATG12.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mouse	√
Rat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Antiserum Preparation	Antiserum to human ATG12 was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.02% Sodium Azide (NaN3)
Immunogen	Synthetic peptide corresponding to a 15 aa sequence from near the centre of human ATG12.
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin¹	√	2.5 ug/ml
Western Blotting	√	1.0 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Histology Positive Control TissueHuman brain Western BlottingAHP1891 detects a band of approximately 40 kDa in mouse heart tissue lysate.
数据
Western blot analysis of mouse heart lysate probed with Rabbit anti Human ATG12 (AHP1891) im the absence (A) or presence (B) of blocking peptide. Immunohistochemical staining of human brain tissue with Rabbit anti Human ATG12 (AHP1891).
相关试剂
Description	Product Code	Applications	Pack Size
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB	0.5 mL

技术参数

产品应用 P*;WB

产品类型 Polyclonal Antibody

推荐用于 P*;WB

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Bio-Rad AHP1891 ATG12 (Internal Region),Rabbit anti ATG12 (Internal Region) antibody

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