Bio-Rad AHP1538 5-lipoxygenase (pSer523),Rabbit anti 5-lipoxygenase (pSer523) antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1538 5-lipoxygenase (pSer523),Rabbit anti 5-lipoxygenase (pSer523) antibody

2024-10-22

Rabbit anti 5-lipoxygenase (pSer523) antibody

货号：AHP1538

规格：0.1 ml

价格：6095

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：5-lipoxygenase (pSer523)

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti 5-lipoxygenase (pSer523) antibodyrecognizes 5-lipoxygenase (5-LO), also known as arachidonate 5-lipoxygenase, when phosphorylated at serine 523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LTs) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LTs include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Antiserum Preparation	Antisera to phosphorylated 5-lipoxygenase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution	10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers	0.09%Sodium Azide 0.01%Bovine Serum Albumin 50%Glycerol
Immunogen	Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes the phosphorylated residue Ser523.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Western Blotting	√		1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Western Blotting AHP1538 detects a doublet of approximately 80kDa in rat cortex lysate.
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数据
Western blot of rat hippocampal lysate showing phospho-specific immunolabelling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523using Rabbit anti 5-lipoxygenase (pSer523) antibody (AHP1538).

技术参数

产品应用 WB

产品类型 Polyclonal Antibody

推荐用于 WB

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Bio-Rad AHP1538 5-lipoxygenase (pSer523),Rabbit anti 5-lipoxygenase (pSer523) antibody

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