Bio-Rad AHP1465 SMAD3,Rabbit anti Human SMAD3 antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1465 SMAD3,Rabbit anti Human SMAD3 antibody

2024-10-22

Rabbit anti Human SMAD3 antibody

货号：AHP1465

规格：50 µg

价格：7718

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原： SMAD3

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Human SMAD3 antibodyrecognizes Mothers against decapentaplegic homolog 3 (SMAD3), a 50 kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase,SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. SMAD3 activity induces differentiation of selected neuronal subtypes at the expense of other subtypes.Defects in SMAD3 may be a cause of colorectal cancer (Sjöblomet al.2006).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Antiserum Preparation	Antiserum to human SMAD3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Immunogen	Synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/25000	1/100000
Immunohistology - Paraffin¹	√		2.5ug/ml
Western Blotting	√	1/2000	1/20000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Human skin
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技术参数

产品应用 ELISA;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;IHC(P)*;WB

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Bio-Rad AHP1465 SMAD3,Rabbit anti Human SMAD3 antibody

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