Rabbit anti Rat MEK 5 (pSer311/pThr315) antibody AHP1302 bio-rad,Rabbit anti Rat MEK 5 (pSer311/pThr315) antibody

2024-10-22

Rabbit anti Rat MEK 5 (pSer311/pThr315) antibody

货号:AHP1302

规格:0.1 ml

价格:6095

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:MEK 5 (pSer311/pThr315)

物种:大鼠

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Rat MEK 5 (pSer311/pThr315) antibodyrecognizes MAPK/ERK kinase 5 (MEK 5), also known as dual specificity mitogen-activated protein kinase kinase 5 (MAPKK 5), when phosphorylated at serine 311 and threonine 315.MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the serine 311 and threonine 315 sites has been implicated in cell proliferation.
产品详情
Target SpeciesRat
Product FormPurified IgG - liquid
Antiserum PreparationAntisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution10mM HEPES pH7.5, 150mM NaCl
ImmunogenSynthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes the phoshorylated residues Ser311 and Thr315.
Approx. Protein ConcentrationsIgG concentration 0.5mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Cameron, S.J.et al.(2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.J Biol Chem. 279 (2): 1506-12.

技术参数

产品应用 wb

产品类型 Polyclonal Antibody

推荐用于 wb

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