| 产品介绍 | | |
| Rabbit anti NFkB p65 (pSer536) antibodyrecognizes nuclear factor NF-kappa-B p65 subunit (NFkB p65), also known as nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 and transcription factor p65, when phosphorylated at serine 536. NFkB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NFkB1) subunits. Other subunits, including p52 (NFkB2), cRel, and RelB exist.NFkB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation.Phosphorylation of serine 536 contributes to NFkB transactivation and increases p65 transcription activity. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to NFkB p65 (pSer536) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Synthetic peptide corresponding to residues surrounding phosphoserine 536 of human NFkB p65 protein. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/500 | 1/3000 |
Immunohistology - Paraffin | √ | 1/50 | 1/250 |
Western Blotting | √ | 1/100 | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human kidney distal tubules and collecting ducts.
- Western Blotting
- AHP1342 detects a band of approximately 65kDa in the appropriate cell lysate.
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| 数据 |
| Published customer image: Rabbit anti NFkB p65 (pSer536) antibody (AHP1342) used to evaluate NF-κB levels in murine hypopharyngeal primary cells (MHPC) lysates by Western blotting. NB: note incorrect citation of p65 S529 in image caption, should read S536.Image caption:Pre- or post-application of ΒΑΥ 11-7082 inhibits acidic bile-induced NF-κB activation and bcl-2 overexpression in MHPC.Western blot analysis is performed in nuclear and cytoplasmic protein extracts of MHPC for p-NF-κB (p65 S529), and cytoplasmic bcl-2. MHPC exposed to acidic bile demonstrate a significant overexpression of nuclear p-p65 and cytoplasmic bcl-2, compared to controls. Pre- or post-application of BAY 11-7082 results in significantly (A-a) reduced nuclear p-p65 levels compared to acidic bile alone. Pre-application also results in significantly reduced (A-b) cytoplasmic p-p65 levels compared to acidic bile alone. However, post-application of BAY 11-7082 results in elevated cytoplasmic p-p65 levels compared to acidic bile alone. (B) Pre- or post-application of BAY 11-7082 results in significant reduction of cytoplasmic bcl-2 levels compared to acidic bile alone (Paired t-test, *p <0.05; **p <0.005; ***p <0.0005; GraphPad Prism 7.0). (Mean ± SD of three independent experiments). (β-actin and Histone 1 are used for the normalization of cytoplasmic and nuclear protein extracts, respectively).From: Doukas PG, Vageli DP, Doukas SG, Sasaki CT.Temporal characteristics of NF-κB inhibition in blocking bile-induced oncogenic molecular events in hypopharyngeal cells.Oncotarget. 2019 May 21;10(36):3339-3351.doi: 10.18632/oncotarget.26917.This image is from an open access article distributed under terms of aCreative Commons Attribution License. |
| 文献 |
| References for NFkB p65 (pSer536) antibody1. Doukas, P.G.et al.(2019) Temporal characteristics of NF-κB inhibition in blocking bile-induced oncogenic molecular events in hypopharyngeal cells.Oncotarget. 10 (36): 3339-51.2. Doukas, S.G.et al.(2020) Thein vivo.preventive and therapeutic properties of curcumin in bile reflux-related oncogenesis of the hypopharynx.J Cell Mol Med. 24 (18): 10311-21.3. Vageli, D.P.et al.(2021) Targeting STAT3 prevents bile reflux-induced oncogenic molecular events linked to hypopharyngeal carcinogenesis.J Cell Mol Med. Dec 01 [Epub ahead of print].Further Reading1. Mattioli, I.et al.(2004) Transient and selective NF-kappa B p65 serine 536 phosphorylation induced by T cell costimulation is mediated by I kappa B kinase beta and controls the kinetics of p65 nuclear import.J Immunol. 172 (10): 6336-44. |
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