Rabbit anti Human FGF basic polyclonal antibody AHP1038B bio-rad,Rabbit anti Human FGF basic polyclonal antibody

2024-10-22

Rabbit anti Human FGF basic polyclonal antibody

货号:AHP1038B

规格:50 µg

价格:3963

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human FGF basic polyclonal antibodyrecognizes human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues.FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135- S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody.
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Biotin - lyophilized
ReconstitutionReconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum PreparationAntisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present
ImmunogenRecombinant human FGF basic (PHP105)
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution
RegulatoryFor research purposes only
Guarantee6 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.15ug/ml0.30ug/ml
Functional Assays0.10.20 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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