Goat anti Rat Leptin antibody AAR40 bio-rad,Goat anti Rat Leptin antibody

2024-10-22

Goat anti Rat Leptin antibody

货号:AAR40

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Leptin

物种:大鼠

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Goat anti Rat Leptin antibodyspecifically recognizes rat leptin, a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. Human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
产品详情
Target SpeciesRat
Product FormPurified IgG - lyophilized
ReconstitutionReconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum PreparationAntiserum to rat Leptin were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present.
ImmunogenRecombinant rat leptin.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml after reconstitution
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0ug/ml
Functional Assays0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Rabbit anti Goat IgG (Fc):FITCSTAR122FF100 Tests
Rabbit anti Goat IgG (Fc):HRPSTAR122PC,E,WB100 Tests

技术参数

产品应用 wb;elisa

产品类型 Polyclonal Antibody

推荐用于 wb;elisa

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