Bio-Rad AHP1076 STAT3,Goat anti Mouse STAT3 antibody

2024-10-22

Goat anti Mouse STAT3 antibody

货号:AHP1076

规格:0.1 mg

价格:5083

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:STAT3

物种:小鼠

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Goat anti Mouse STAT3 antibodyrecognizes an epitope within the C-terminal (CT) region of signal transducer and activator of transcription 3 (STAT3), an 80-85 kDa member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus.STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human STAT3 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%Sodium Azide

0.5%Bovine Serum Albumin

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Immunogen

Peptide sequence DMELTSECARSPM corresponding to the C-terminal region of STAT3 (NP_003141.2; NP_644805.1).

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/32,000

Immunohistology - Frozen1

10ug/ml
Immunohistology - Paraffin

Western Blotting

0.55ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

1Overnight staining is recommended for this antibodyWhere this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Histology Positive Control Tissue
  • Mouse Thymus. .
  • Western Blotting
  • Goat anti STAT3 antibody detects a band of approximately 80-85kDa in A431 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
二抗相关产品

Description

Product Code

Applications

Pack Size

Rabbit anti Goat IgG (Fc):FITCSTAR122FFlow1 mg
Rabbit anti Goat IgG (Fc):HRPSTAR122PC;E;WB1 mg
数据

Immunohistochemical staining of paraffin embedded mouse thymus using Goat anti STAT3 antibody (AHP1076) at 4μgml-1 following heat mediated antigen retrieval with Tris/EDTA buffer pH 9, antibody visualization is by HRP-staining and DAB development
文献

1. Levy, D.E. & Lee, C.K. (2002) What does Stat3 do?J Clin Invest. 109 (9): 1143-8.

技术参数

产品应用 IHC(F)*;ELISA;IHC(P);WB

产品类型 Polyclonal Antibody

推荐用于 IHC(F)*;ELISA;IHC(P);WB

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