Bio-Rad AHP1069 Osteopontin,Goat anti Human Osteopontin antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1069 Osteopontin,Goat anti Human Osteopontin antibody

2024-10-22

Goat anti Human Osteopontin antibody

货号：AHP1069

规格：0.1 mg

价格：5083

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Osteopontin

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

产品介绍
Goat anti Human Osteopontin antibodyrecognises an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated ~60 kDa glycoprotein. Osteopontin is a secreted chemokine-like protein and member of the SIBLING family, and is synthesised by many cell types.Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid, ready to use.
Antiserum Preparation	Antisera to osteopontin (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution	TRIS buffered saline
Preservative Stabilisers	0.02%Sodium Azide (NaN₃) 0.5%Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.5mg/ml
Immunogen	Peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		1/ 64000
Western Blotting	√	1.0	3.0 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Western Blotting AHP1069 detects a band of approximately 60kDa in lysates of human thymus, tonsil and spleen. Approximate 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glcoprotein.
二抗相关产品
Description	Product Code	Applications	Pack Size
Rabbit anti Goat IgG (Fc):FITC	STAR122F	Flow	1 mg
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C;E;WB	1 mg
数据
Western blotting analysis of MOLT4 lysate (35µg protein in RIPA buffer) using Goat anti Human osteopontin antibody (AHP1069) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
文献
1. Rangaswami, H.et al. (2006) Osteopontin: role in cell signaling and cancer progression.Trens Cell Biol. 16: 79-87.

技术参数

产品应用 ELISA;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;WB

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Bio-Rad AHP1069 Osteopontin,Goat anti Human Osteopontin antibody

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