Bio-Rad AHP1043 MIP-1 beta,Rabbit anti Human MIP-1 beta antibody

2024-10-22

Rabbit anti Human MIP-1 beta antibody

货号:AHP1043

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:MIP-1 beta

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human MIP-1 beta antibodyrecognizes human MIP-1 beta, otherwise known as CCL4, a 69 amino acid member of the CC chemokine family, shown to signal through the chemokine receptors CCR1, CCR2 and CCR5.MIP-1 beta is involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as monocytes, B cells, NK cells, neutrophils, dendritic cells and CD4+ lymphocytes.The secretion of MIP-1 beta by CD8+ lymphocytes has been shown to act as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry.
产品详情

Target Species

Human

Product Form

Purified IgG - lyophilized

ReconstitutionReconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum PreparationAntisera to human MIP-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

None present

Carrier FreeYes

Immunogen

Recombinant human MIP-1 beta.

Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.5ug/ml
Functional Assays1.753.0ug/ml
Immunohistology - Paraffin 1

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
References for MIP-1 Beta antibody
  1. Cocchi, F.et al.(1995) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.Science. 270 (5243): 1811-5.
Further Reading
  1. Menten, P.et al.(2002) Macrophage inflammatory protein-1.Cytokine Growth Factor Rev. 13 (6): 455-81.

技术参数

产品应用 E;FN;P*;WB

产品类型 Polyclonal Antibody

推荐用于 E;FN;P*;WB

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