| 产品介绍 | | |
| Rabbit anti Human CD193 antibodyrecognizes human CD193, otherwise known as CC chemokine receptor 3 (CCR3), a seven-helix transmembrane G-protein coupled receptor which, along with CCR5 and CXCR4, acts as a principal receptor for the fusion and entry of HIV into target cells. CD193 is expressed by eosinophils, basophils, mast cells, microglia, Th2helper cells and some epithelial cells.CD193 acts as a receptor for several CC chemokines including monocyte chemoattractant proteins (MCP) -1, -2, -3 and -4, MIP-1a, MIP-1β, and RANTES. The eotaxin subgroup of CC chemokines act as specific agonists for CD193 involved in infection of microglia by HIV (Heet al.1997) and allergic reactions (Holseet al.2006).Rabbit anti Human CD193 antibody has been used successfully for the detection of human CD193 by immunohistochemistry on formalin fixed, paraffin embedded intervertebral disc tissue (Gruberet al.2014). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human CD193 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1 mg/ml |
Immunogen | Synthetic peptide corresponding to a 14 amino acid sequence located near human CD193 carboxy-terminus. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | | |
Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin1 | √ | | 10ug/ml |
Western Blotting | √ | | 1ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Histology Positive Control TissueHuman spleen.Western BlottingAHP1010 detects a band of approximately 55kDa in human spleen cell lysates (predicted Mwt. 41kDa) |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
| Formalin fixed, paraffin embedded normal human spleen stained with Rabbit anti Human CD193 (AHP1010) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). |
| 文献 |
| References for CD193 antibody1. Wainwright, D.A.et al.(2009) Exacerbation of facial motoneuron loss after facial nerve axotomy in CCR3-deficient mice.ASN Neuro. 1 (5): e00024.2. Kallankari, H.et al.(2010) Chemokine CCL18 predicts intraventricular hemorrhage in very preterm infants.Ann Med. 42: 416-25.3. Gruber, H.E.et al.(2014) Production and expression of RANTES (CCL5) by human disc cells and modulation by IL-1-β and TNF-α in 3D culture.Exp Mol Pathol. 96: 133-8.Further Reading1. Holse, M.et al.(2006) CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals.Clin Mol Allergy. 4: 6.2. Combadiere, C.et al.(1996) Cloning and functional expression of a human eosinophil CC chemokine receptor.J Biol Chem. 271 (18): 11034.3. He, J.et al.(1997) CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia.Nature. 385 (6617): 645-9. |
QQ:1749072012