Bio-Rad AAM62B MCP-2 ,Rabbit anti Mouse MCP-2: Biotin

2024-10-22

Rabbit anti Mouse MCP-2: Biotin

货号:AAM62B

规格:50 µg

价格:4055

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:MCP-2

物种:小鼠

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍
Rabbit anti Mouse MCP-2 antibodyrecognizes mouse MCP-2 (Monocyte Chemoattractant Protein 2), otherwise known as CCL8, a CC chemokine originally isolated from the human osteosarcoma cell line MG-63, which is expressed at highest levels in peripheral blood cells and the small intestine.MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, and signals through binding to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional CC chemokine inhibitor, which may be important during inflammatory responses.
产品详情

Target Species

Mouse

Product Form

Purified IgG conjugated to Biotin - lyophilized

Antiserum Preparation

Antiserum to mouse MCP-2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Approx. Protein Concentrations

IgG concentration 0.1mg/ml after reconstitution

Immunogen

Recombinant mouse MCP-2.

Regulatory

For research purposes only

Guarantee

Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Reconstitution

Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.25

1.0ug/ml

Western Blotting

0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 ELISA;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;WB

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