Mouse anti Human lamin A/C, clone JOL2 伯乐 MCA1429GA Lamin A/C 抗体

2024-10-29

Mouse anti Human lamin A/C, clone JOL2

货号:MCA1429GA

规格:0.1 mg

市场价格:5398

产品类型:流式抗体

品牌:Bio-Rad

抗原:Lamin A/C

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号: JOL2

荧光染料:Purified

产品介绍
Mouse anti Human lamin A/C, clone JOL2recognizes both recombinant and native forms of human lamin A and C, nuclear intermediate filament proteins encoded by the same LMNA gene via alternative splicing. In addition to providing structural support to the nuclear envelope, these proteins contribute to chromatin organization and the regulation of gene expression.Mutations in the LMNA gene are responsible for a broad spectrum of disorders, termed laminopathies, including Emery-Dreifuss muscular dystrophy and dilated cardiomyopathy.Mouse anti Human lamin A/C, clone JOL2 has been shown to bind to an epitope between amino acids 464-572.
产品详情

Target Species

Human

Species Cross-ReactivityTarget SpeciesCross Reactivity
Xenopus
N.B. Antibody reactivity and working conditions may vary between species.
Product FormPurified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

ImmunogenRecombinant human lamin A
Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2-0/Ag4 myeloma cell line

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 10.4 ug/ml
Immunoprecipitation
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.
  1. 1Mouse anti lamin A/C requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer pH 6.2 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Histology Positive Control Tissue
  • Human colon
数据

Formalin fixed, paraffin embedded section of human colon stained with Mouse anti Lamin A/C (MCA1429GA).
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative ControlMCA928F0.1 mg
文献
1. Tilgner, K.et al.(2009) Dynamic complexes of A-type lamins and emerin influence adipogenic capacity of the cell via nucleocytoplasmic distribution of beta-catenin.J Cell Sci. 122: 401-13.2. Ivorra, C.et al.(2006) A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C.Genes Dev. 20: 307-20.3. Navarro, C.L.et al.(2004) Lamin A and ZMPSTE24 (FACE-1) defects cause nuclear disorganization and identify restrictive dermopathy as a lethal neonatal laminopathy.Hum Mol Genet. 13: 2493-503.4. Cummings, M.et al.(2006) XPA versus ERCC1 as chemosensitising agents to cisplatin and mitomycin C in prostate cancer cells: role of ERCC1 in homologous recombination repair.Biochem Pharmacol. 72: 166-75.5. Markiewicz, E.et al.(2006) The inner nuclear membrane protein emerin regulates beta-catenin activity by restricting its accumulation in the nucleus.EMBO J. 25: 3275-85.6. Zhang, Q.et al.(2007) Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity.Hum Mol Genet. 16: 2816-33.7. Jean, D.et al.(2008) Cathepsin L expression is up-regulated by hypoxia in human melanoma cells: role of its 5'-untranslated region.Biochem J. 413: 125-34.8. Ndong, Jde. L.et al.(2009) Down-regulation of the expression of RB18A/MED1, a cofactor of transcription, triggers strong tumorigenic phenotype of human melanoma cells.Int J Cancer. 124: 2597-606.

技术参数

产品应用 IHC;IF;Flow;IP

产品类型 Monoclonal Antibody

推荐用于 IHC;IF;Flow;IP

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