Bio-Rad AAM51B VEGF,Rabbit anti Mouse VEGF:Biotin

2024-10-22

Rabbit anti Mouse VEGF:Biotin

货号:AAM51B

规格:50 µg

价格:4055

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:VEGF

物种:小鼠

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍
Rabbit anti Mouse VEGF antibodyrecognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth.Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species.
产品详情

Target Species

Mouse

Product Form

Purified IgG conjugated to Biotin - lyophilized

Antiserum Preparation

Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml after reconstitution

Immunogen

Recombinant mouse VEGF

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Reconstitution

Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.25ug/ml

1.0ug/ml

Immunohistology - Paraffin

Western Blotting

0.10ug/ml0.20ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
  • ELISA
  • This antibody may be used in direct ELISA or as a detection reagent in a sandwich ELISA withAAM51as a capture reagent andPMP67as the standard.
文献

1. Nahrendorf, M.et al.(2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.J Exp Med. 204: 3037-47.2. Araújo, W.F.et al.(2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.Urol Oncol. 33 (9): 389.e1-7.3. Lin, E.Y.et al.(2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.Mol Oncol. 1: 288-302.4. HogenEsch Het al.(2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.Exp Mol Pathol. Oct 26. pii: S0014-4800(16)30084-3. [Epub ahead of print]5. Hogenesch, H.et al.(2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.Exp Mol Pathol. 101 (3): 303-7.

技术参数

产品应用 ELISA;IHC(P);WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;IHC(P);WB

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