Bio-Rad AAM51 VEGF,Rabbit anti Mouse VEGF-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AAM51 VEGF,Rabbit anti Mouse VEGF

2024-10-22

Rabbit anti Mouse VEGF

货号：AAM51

规格：0.1 mg

价格：4063

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：VEGF

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Mouse VEGF antibodyrecognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth.Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species.
产品详情
Target Species	Mouse
Product Form	Purified IgG - lyophilized
Antiserum Preparation	Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml after reconstitution.
Immunogen	Recombinant mouse VEGF
Regulatory	For research purposes only
Guarantee	6 months from date of despatch
Reconstitution	Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		0.5ug/ml
Functional Assays	√	1.0ug/ml	2.0ug/ml
Immunofluorescence	√	2.5ug/ml
Immunohistology - Paraffin¹	√	2.5ug/ml
Western Blotting	√	0.1ug/ml	0.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. ELISA This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA withAAM51Bas a detection reagent andPMP67as the standard. Histology Positive Control Tissue Kidney
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文献
1. Nahrendorf, M.et al.(2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.J Exp Med. 204: 3037-47.2. Araújo, W.F.et al.(2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.Urol Oncol. 33 (9): 389.e1-7.3. Lin, E.Y.et al.(2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.Mol Oncol. 1: 288-302.4. HogenEsch Het al.(2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.Exp Mol Pathol. Oct 26. pii: S0014-4800(16)30084-3. [Epub ahead of print]5. Hogenesch, H.et al.(2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.Exp Mol Pathol. 101 (3): 303-7.

技术参数

产品应用 ELISA;FN;IF;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;FN;IF;IHC(P)*;WB

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Bio-Rad AAM51 VEGF,Rabbit anti Mouse VEGF

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