Bio-Rad AAM48 MIP-2,Rabbit anti Mouse MIP-2-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AAM48 MIP-2,Rabbit anti Mouse MIP-2

2024-10-22

Rabbit anti Mouse MIP-2

货号：AAM48

规格：0.1 mg

价格：4063

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：MIP-2

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Mouse MIP-2 antibodyrecognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
产品详情
Target Species	Mouse
Product Form	Purified IgG - lyophilized
Antiserum Preparation	Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml after reconstitution.
Immunogen	Recombinant mouse MIP-2
Regulatory	For research purposes only
Guarantee	6 months from date of despatch
Reconstitution	Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		0.5ug/ml
Functional Assays	√	0.50ug/ml	0.90ug/ml
Immunohistology - Frozen	√
Immunohistology - Paraffin¹	√
Western Blotting	√	0.1ug/ml	0.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.ELISARabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody (AAM48B) as the detection antibody and recombinant Mouse MIP-2 protein (PMP55) as a standard.Western BlottingRabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 (PMP55) protein as a positive control.
二抗相关产品
Description	Product Code	Applications	Pack Size
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E;WB	1 mg
Goat anti Rabbit IgG (Fc):FITC	STAR121F	Flow	1 mg
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E;WB	1 mg
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C;E;WB	1 mg
Sheep anti Rabbit IgG:FITC	STAR34B	C;F	1 mg
文献
1. Tittel, A.P.et al.(2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.J Am Soc Nephrol. 22 (8): 1435-41.2. Roche, J.K.et al.(2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.Am J Pathol. 170: 526-37.3. Di Carlo, E.et al.(2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.J Immunol. 165: 3111-8.4. Cavallo, F.et al(2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.Cancer Res. 61: 3518-23.5. González-López, A.et al.(2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.Am J Physiol Lung Cell Mol Physiol. 301: L500-9.6. Lazic, M.et al.(2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.J Hepatol. 61: 107-15.7. Nicholas, J.et al.(2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.Innate Immun. 21 (3): 266-74.8. Yoshida, T.et al.(2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.Genes Cells. 19: 842-52.

技术参数

产品应用 IHC(F);ELISA;FN;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 IHC(F);ELISA;FN;IHC(P)*;WB

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Bio-Rad AAM48 MIP-2,Rabbit anti Mouse MIP-2

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