Rabbit anti Mouse TNF alpha antibody bio-rad,Rabbit anti Mouse TNF alpha antibody

2024-10-22

Rabbit anti Mouse TNF alpha antibody

货号:AAM19GA

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:TNF alpha

物种:小鼠

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Mouse TNF alpha antibodyrecognizes mouse Tumour Necrosis Factor alpha (TNF–alpha), a 17.3kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. This Rabbit anti Mouse TNF-alpha antibody can be used to neutralize the effects of mouse TNF-alpha.
产品详情
Target SpeciesMouse
Product FormPurified IgG - liquid
ReconstitutionReconstitute with 0.1 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present
ImmunogenRecombinant mouse TNFα(PMP24)
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml after reconstitution.
RegulatoryFor research purposes only
Guarantee6 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0ug/ml
Functional Assays0.010.015ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Khaskhely, N.M.et al.(2002) Low-dose UVB contributes to host resistance against Leishmania amazonensis infection in mice through induction of gamma interferon and tumor necrosis factor alpha cytokines.Clin Diagn Lab Immunol. 9: 677-86.2.Schafers, M.et al.(2002) Anterograde transport of tumor necrosis factor-alpha in the intact and injured rat sciatic nerve.J Neurosci. 22: 536-45.3.Oakley, F.et al.(2005) Nuclear factor-kappaB1 (p50) limits the inflammatory and fibrogenic responses to chronic injury.Am J Pathol. 166: 695-708.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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