| 产品介绍 | | |
| Rabbit anti Mouse MCP-1 antibodyrecognizes mouse monocyte chemotactic protein-1 (MCP-1), also known as CCL2, Small-inducible cytokine A2 or Platelet-derived growth factor-inducible protein JE. Mouse MCP-1 is a 148 amino acid ~16 kDa secreted monocyte chemotactic factorThe chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages (Conti and DiGioacchino 2001). |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | Recombinant mouse MCP-1 (PMP35) |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
Functional Assays | √ | 4.0 | 6.0ug/ml |
| Immunofluorescence | √ | | |
Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAThis product may be used in an indirect ELISA together withPMP35as the standard. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
| Published customer image:Rabbit anti Mouse MCP-1 antibody (AAM43) used for the identification of MCP-1 expressing cells in murine glioma by indirect immunofluorescence on formailin fixed vibratome sections.Image caption:CCL2+cells are GFAP+astrocytes in human glioma.(A) Confocal images show co-localization of CCL2-expressing cells (red) with GFAP (green) in glioma. Insert shows a detail of a single CCL2-expressing cell (red) co-localizing with GFAP (green) and counterstained with DAPI (blue). Scale bar: 30 μm. (B) Areas of infiltration of CD3+T-cells (green) coincide with the areas of CCL2-expressing cells (red) in glioma. Confocal images show a tumorigenic area, infiltrated with CD3+T-cells (green) in an area with numerous CCL2-expressing cells (red). The immunostaining was combined with a counterstaining with DAPI to detect the nucleus (blue). Scale bar: 50 μm. (C) Examples of characteristic CD3 infiltration in samples of glioma. CD3+T-cells can be observed grouped in BVs (BV, limited by broken red line in 1 and 3) but also infiltrated in the parenchyma (2, 4, blue insert magnified in 5). (D) CCL2 expression correlates with the infiltration of T-cells in the tumor areas. The quantification in serial sections of the number of T-cells, either infiltrated or located in the BV lumen, revealed that the level of infiltration of CD3+T-cells in the parenchyma (green) is positively correlated with the level of CCL2 expression (BV; BV lumen).From: Citation: Carrillo-de Sauvage MA, Gómez A, Ros CM, Ros-Bernal F, Martín ED, Perez-Vallés A, et al. (2012)CCL2-Expressing Astrocytes Mediate the Extravasation of T Lymphocytes in the Brain. Evidence from Patients with Glioma and Experimental ModelsIn Vivo.PLoS ONE 7(2): e30762. doi:10.1371/journal.pone.0030762.This image is from an open access article distributed under the terms of theCreative Commons Attribution License. |
| 文献 |
| 1. Tesch GHet al.(1999) Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis.J Clin Invest. 103:73-802. Ruan, L.et al.(2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.Curr Alzheimer Res. 6: 531-40.3. Carrillo-de Sauvage, M.A.et al.(2012) CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental modelsin vivo.PLoS One. 7: e307624. Ji, Z.et al.(2014) Thiamine Deficiency Promotes T Cell Infiltration in Experimental Autoimmune Encephalomyelitis: The Involvement of CCL2.J Immunol. 193: 2157-67.5. Zhang, L.et al.(2012) Intra-amniotic administration of E coli lipopolysaccharides causes sustained inflammation of the fetal skin in sheep.Reprod Sci. 19 (11): 1181-9. |
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