Bio-Rad RAB8A抗体 AHP1683,Goat anti Human RAB8A (C-Terminal)

2024-10-22

Goat anti Human RAB8A (C-Terminal)

货号:AHP1683

规格:0.1 mg

价格:5083

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原: RAB8A

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍

Goat anti Human RAB8A antibodyrecognizes an epitope within the C-terminal (CT) region of the human RAS superfamily member Rab8a (Ras-related protein Rab-8A), otherwise known as oncogene c-mel, Rab8A is a small monomeric GTP/GDP-binding protein with a predicted molecular weight of ~24 kDa which may play a role in vesicular trafficking and neurotransmitter release.The Rab family of proteins, originally described asRas-like proteins inbrain are the largest group of GTPases within the Ras superfamily with 61 protein members identified. Rab family members are involved in the regulation of intracellular vesicular transport and movement of proteins between endocytic and secretory organelles (Wennerberget al.2005). Rab8A is involved in vesicular traffic between the trans golgi network and the basoleteral aspect of the plasma membrane (Huberet al.1993).Goat anti human Rab8a antibody has been successfully used for the detection of Rab8a in human intestinal epithelial cells using western blotting (Vaccaet al.2014).

产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity

Dog Expected from Sequence

Bovine Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

AntiserumPreparation

Antiserum to human RAB8A (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin

Immunogen

Peptide sequence C-NQGVKITPDQQKR from the C-Terminal region of RAB8A (NP_005361.2).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/32000

Western Blotting

0.1

0.3ug/ml

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • AHP1683 detects a band of approximately 25kDa in human cerebellum and kidney cell lysates

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文献

1.Vacca, B.et al.(2014) Drebrin E depletion in human intestinal epithelial cells mimics Rab8a loss of function.

Hum Mol Genet. 23 (11): 2834-46.

技术参数

产品应用 E;WB

产品类型 Polyclonal Antibody

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