Native Bovine Ubiquitin bio-rad 9400-1502,Native Bovine Ubiquitin

2024-10-22

Native Bovine Ubiquitin

货号:9400-1502

规格:25 mg

价格:3613

产品类型:蛋白

品牌:Bio-Rad

产品介绍
Ubiquitin is a protein modifier that is covalently linked to target lysines and functions in protein degradation.
产品详情
Target SpeciesBovine
Product FormPurified protein from bovine erythrocytes - lyophilized
ReconstitutionReconstitute with 25 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preservative StabilisersNone present
Purity>98% by SDS PAGE
Approx. Protein Concentrations1.0 mg/ml after reconstitution
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/501/500
Western Blotting1/2501/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Wilkinson, K.D. & Audhya, T.K. (1981) Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly.J Biol Chem. 256 (17): 9235-41.2.Levinger, L. & Varshavsky, A. (1982) Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.Cell. 28 (2): 375-85.3.Ciechanover, A.et al.(1984) The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation.J Cell Biochem. 24 (1): 27-53.4.Haas, A.L. & Bright, P.M. (1985) The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.J Biol Chem. 260 (23): 12464-73.5.Yang, C.S.et al.(2017) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.Mol Cell. 66 (4): 503-516.e5.

技术参数

产品应用 elisa

产品类型 Purified Protein

推荐用于 elisa

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