Bio-Rad AAI23F IgG,Sheep anti Bovine IgG:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AAI23F IgG,Sheep anti Bovine IgG:FITC

2024-10-22

Sheep anti Bovine IgG:FITC

货号：AAI23F

规格：1 mg

价格：2695

产品类型：荧光二抗

品牌：Bio-Rad

抗原： IgG

物种：Bovine

宿主：Sheep

抗体亚型：IgG

荧光染料：FITC

产品介绍
Sheep anti Bovine IgG polyclonal antibodyrecognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other speciesSheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrielinget al.2013).
产品详情
Target Species	Bovine
Product Form	Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation	Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Immunogen	Purified bovine IgG.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
Immunohistology - Frozen	√	1/200	1/2000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Somda, M.B.et al.(2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.Parasit Vectors. 9 (1): 149.2. Facciuolo, A.et al.(2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches toMycobacterium aviumsubsp.paratuberculosisSecreted Proteins following Targeted Enteric Infection in Young Calves.PLoS One. 11 (7): e0158747.3. Subharat, S.et al.(2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.Vet Immunol Immunopathol. 164 (3-4): 201-7.4. Benedictus, L.et al.(2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.Vaccine. 34 (8): 1002-5.5. Denholm, S.J.et al.(2018) Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements.J Dairy Sci. 101 (11): 10248-10258.6. Alo, Ket al.(2018) Passive protective effect of anti-K99 antibodies against enterotoxigenicE.coli.infection in neonatal calvesIr J of Vet Med, 12(2), 97-1077. Somda, M.B.et al.(2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.Vet Parasitol. 197 (1-2): 332-40.8. Waap, H.et al.(2011) A modified agglutination test for the diagnosis ofBesnoitia besnoiti.infection.Vet Parasitol. 178 (3-4): 217-22.9. Springer, A.et al.(2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine LungwormDictyocaulus viviparus..Pathogens 2022, 11, 55.10. Anastácio, C.et al.(2022) Impact of Endemic Besnoitiosis on the Performance of a Dairy Cattle Herd.Animals (Basel). 12 (10): 1291.

技术参数

产品应用 C;F

产品类型 Polyclonal Antibody

推荐用于 C;F

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Bio-Rad AAI23F IgG,Sheep anti Bovine IgG:FITC

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