产品介绍 |
| Rabbit anti Human CD282 antibodyrecognises CD282, also known as TLR-2, a ~90 kDa type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. CD282 is also expressed in the spleen, bone marrow and lymph nodes. CD282 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target SpeciesCross Reactivity |
| Mouse √ |
| Horse √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antisera to Human CD282 (NT) were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Immunogen | A peptide corresponding to an 14 amino acid sequence from near the amino terminus of human CD282. |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | 2.0ug |
Western Blotting | √ | 0.5 | 1.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human spleen
- Western Blotting
- AHP1635 detects a band of approximately 100kDa in A-20 cell lysate
|
可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
相关产品 |
Description | Product Code | Applications | Pack Size |
Antigen Retrieval Buffer, pH8.0 | BUF025A | p | 500 ml |
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
文献 |
| 1.Siemieniuch MJet al.(2016) Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares.PLoS One. 11 (5): e0154934.2.Vogel, S.N.et al.(2003) TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression.Mol Interv. 3 (8): 466-77.3.Takeda, K.et al.(2003) Toll-like receptors.Annu Rev Immunol. 21: 335-76.4.Janeway, C.A. Jr. & Medzhitov, R. (2002) Innate immune recognition.Annu Rev Immunol. 20: 197-216.5.O'Neill, L.A.J,et al(2003) The Toll-IL-1 receptor adaptor family grows to five members.Trends in Imm. 24:286-9. |
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