| 产品介绍 | | |
| Sheep anti Human Neuropeptide Y Receptor 1 antibodyrecognizes human neuropeptide Y receptor 1. Neuropeptide Y receptors are a class of G-protein coupled receptors which are activated by the closely related peptide hormones neuropeptide Y, peptide YY and pancreatic polypeptide. These receptors are involved in control of a diverse set of behavioral processes including appetite, circadian rhythm, and anxiety. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 5.0 mg/ml |
Immunogen | A synthetic peptide corresponding to amino acids 365 - 378 of human NPY1R, conjugated to KLH.Peptide sequence: KQASPVAFKKINNN |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunofluorescence | √ | | |
| Immunohistology - Paraffin1 | √ | | |
Western Blotting | √ | 1/100 | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western Blotting6732-0150 detects a band of approximately 55 kDa in human brain. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;F;WB | 1.5 ml |
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F;IF | 0.1 mg |
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
| 数据 |
| Published customer image:Sheep anti Human neuropeptide Y receptor 1 antibody (6732-0150) used for the detection of NY1R in rat retinal ganglion cells by immunofluorescence.Image caption:.NPY and NPY receptor immunoreactivity (ir) in a purified RGC culture obtained from Wistar pups (a–e, red). RGCs were stained with antiBrn3a (RGC marker, green) and nuclei with DAPI (blue). Scale bar: 20 μm.From: Martins J, Elvas F, Brudzewsky D, Martins T, Kolomiets B, Tralhão P, Gøtzsche CR, Cavadas C, Castelo-Branco M, Woldbye DP, Picaud S, Santiago AR, Ambrósio AF. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiologyand Exerts Neuroprotective ActionsIn Vitro.ASN Neuro. 2015 Aug 26;7(4). pii:1759091415598292.This image is from an open access article distributed under the terms of theCreative Commons Attribution License. |
| 文献 |
| 1. Ferreira, R.et al.(2010) Neuropeptide Y Modulation of Interleukin-1{beta} (IL-1{beta})-induced Nitric Oxide Production in Microglia.J Biol Chem. 285: 41921-34.2. Decressac, M.et al.(2009) Neuropeptide Y stimulates proliferation, migration and differentiation of neural precursors from the subventricular zone in adult mice.Neurobiol Dis. 34: 441-9.3. Martins, J.et al.(2015) Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiology and Exerts Neuroprotective ActionsIn Vitro.ASN Neuro. 7 (4) pii: 1759091415598292.4. Baptista, S.et al.(2012) Neuropeptide Y promotes neurogenesis and protection against methamphetamine-induced toxicity in mouse dentate gyrus-derived neurosphere cultures.Neuropharmacology. 62 (7): 2413-23.5. Thiriet, N.et al.(2011) NPY promotes chemokinesis and neurogenesis in the rat subventricular zone.J Neurochem. 116 (6): 1018-27.6. Ventura, F.et al.(2020) Protective effect of neuropeptide Y2 receptor activation against methamphetamine-induced brain endothelial cell alterations.Toxicol Lett. 334: 53-59. |
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