Mouse anti Human Myeloperoxidase:Alexa Fluor® 647 MCA1757A647 BIO-RAD MCA1757A647 Mouse anti Human Myeloperoxidase:Alexa F

2024-10-29

Mouse anti Human Myeloperoxidase:Alexa Fluor® 647

货号:MCA1757A647

规格:100 Tests/1ml

市场价格:3162

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:2C7

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti Human myeloperoxidase antibody, clone 2C7recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patryet al.2003).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Alexa Fluor 647 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665
ImmunogenHuman myeloperoxidase
Fusion PartnersSpleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
推荐产品
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647F100 Tests
文献
1.Audrain, M.A.et al.(1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.Clin Exp Immunol. 107 (1): 127-34.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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