| 产品介绍 | | |
| Mouse anti Rat Nestin antibody, clone Rat-401recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | TRIS buffered glycine. |
Preservative Stabilisers | 0.05% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | Nestin purified from embryonic rat spinal cord. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Fusion Partners | Spleen cells from immunized Balb/c mice were fused with cells of the NS1 myeloma cell line. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | | |
Immunohistology - Frozen | √ | 1/40 | 1/400 |
| Immunohistology - Paraffin | √ | 1/40 | 1/400 |
Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.ImmunohistologyWe recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.Western BlottingMouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts. For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested. |
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| 文献 |
| Source Reference1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.J Neurosci. 5 (12): 3310-28.References for Nestin antibody1. Arnold, T.D.et al.(2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β.J Neurosci. 32: 1197-206.2. Mori, T.et al.(2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.Mol Cell Biol. 25: 5183-95.3. Choi, J.S.et al.(2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina.J Comp Neurol. 518: 1064-81.4. Choi, J.S.et al.(2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats.J Histochem Cytochem. 55: 821-30.5. Choi, J.S.et al.(2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord.Cell Tissue Res. 337: 27-36.6. Shin, Y.J.et al.(2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats.Neurosci Lett. 469: 194-8.7. Zhang, H.et al.(2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors.J Cell Biol. 163: 1375-84.8. Aleksandrova, M.A.et al.(2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and DifferentiationBull Exp Biol Med. 132: 1000-3.View The Latest Product References |
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