Bio-Rad Mouse anti Pig CD203a, clone PM18-7 ,Mouse anti Pig CD203a, clone PM18-7

2024-10-22

Mouse anti Pig CD203a, clone PM18-7

货号:MCA1973F

规格:0.1 mg

价格:3740

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD203a

物种:pig

宿主:小鼠

抗体亚型:IgG1

克隆号:PM18-7

荧光染料:Purified

产品介绍
Mouse anti Pig CD203a, clone PM18-7recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguezet al.1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 orENPP1(Petersenet al.2007).Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguezet al.1998) under both reducing and non-reducing conditions. CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersenet al.2007) from preparations of porcine alveolar macrophages.CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersenet al.2007,Hwanget al.2015), it is not expressed on monocyte populations (McCulloughet al.1997,Hwanget al.2015).SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchezet al.1999).
产品详情
Target SpeciesPig
Product FormIg Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide
ImmunogenPorcine alveolar macrophages.
Fusion PartnersSpleen cells from immunized mice were fused with P3X63-Ag8-653 murine myeloma cells (Kearneyet al.1979).
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
Approx. Protein ConcentrationsIgG concentration 0.1mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.McCullough, K.C.et al.(1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.Vet Immunol Immunopathol. 58 (3-4): 265-75.2.McCullough, K.C.et al.(1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.Immunology. 98 (2): 203-12.3.Boersma, W.J.et al.(2001) Summary of workshop findings for porcine B-cell markers.Vet Immunol Immunopathol. 80 (1-2): 63-78.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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