Bio-Rad 4737-1010 G Protein Alpha 0,Rabbit anti Mouse G Protein Alpha 0

2024-10-22

Rabbit anti Mouse G Protein Alpha 0

货号:4737-1010

规格:1 ml

价格:3842

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:G Protein Alpha 0

物种:小鼠

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Mouse G Protein alpha 0 antibodyrecognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with β and γ subunits. Two isoforms of the α subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) inDrosophilaby western blotting (Guoet al.2011).
产品详情

Target Species

Mouse

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Approx. Protein Concentrations

IgG concentration 5.0 mg/ml

Immunogen

Synthetic peptide corresponding to the amino acids 345 - 354 (C-ANNLRGCGLY) of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/500

1/1000

Western Blotting1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This antibody detects band at 39 kDa when used under reducing conditions against rat brain.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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数据

Synthetic G Protein Alpha 0 detected with Rabbit anti Mouse G Protein Alpha 0 (4737-1010).
文献

1. Guo, F.et al.(2011) Go signaling in mushroom bodies regulates sleep in Drosophila.Sleep. 34: 273-81.

技术参数

产品应用 ELISA;WB*

产品类型 Polyclonal Antibody

推荐用于 ELISA;WB*

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