Bio-Rad 4670-1725F Glucose Transporter 4,Mouse anti Glucose Transporter 4:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 4670-1725F Glucose Transporter 4,Mouse anti Glucose Transporter 4:FITC

2024-10-22

Mouse anti Glucose Transporter 4:FITC

货号：4670-1725F

规格：0.1 mg

价格：3247

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Glucose Transporter 4

物种：大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：1F8

荧光染料：FITC

产品介绍
Mouse anti glucose transporter 4 antibody, clone 1F8originally raised against rat intracellular low density microsomes (Jameset al.1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (Jameset al.1988).Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetiket al.2014).
产品详情
Target Species	Rat
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN₃)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Immunogen	Partially purified vesicles containing insulin-responsive glucose transporter 4.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
Max Ex/Em	490/525
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence	√	Neat	1/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
数据
HeLa cells stained with Mouse anti Glucose Transporter 4:FITC (4670-1725F) and counterstained with DAPI.
文献
1. James, D.E.et al.(1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.Nature. 333 (6169): 183-5.2. James, D.E.et al.(1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.Nature. 338 (6210): 83-7.3. Cleasby, M.E.et al.(2003) Programming of rat muscle and fat metabolism byin uterooverexposure to glucocorticoids.Endocrinology. 144 (3): 999-1007.4. Huang, J.,et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein.Proc Natl Acad Sci U S A. 98:13084-13089.5. Farese, R.V.et al.(2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.J Clin Invest. 117: 2289-301.6. Grainger, D.L.et al.(2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.Pflugers Arch. 462: 723-32.

技术参数

产品应用 IF

产品类型 Monoclonal Antibody

推荐用于 IF

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Bio-Rad 4670-1725F Glucose Transporter 4,Mouse anti Glucose Transporter 4:FITC

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