Bio-Rad 4520-5306 Alpha Fetoprotein,Mouse anti Human Alpha Fetoprotein-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 4520-5306 Alpha Fetoprotein,Mouse anti Human Alpha Fetoprotein

2024-10-22

Mouse anti Human Alpha Fetoprotein

货号：4520-5306

规格：1 mg

价格：5100

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Alpha Fetoprotein

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：2I9/2 (166)

荧光染料：Purified

产品介绍
Mouse anti Human alpha fetoprotein antibody, clone 219/2recognizes alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a ~68 kDa plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adultsElevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome.Mouse anti Human alpha fetoprotein antibody, clone 219/2 does not cross-react with human albumin and other human serum proteins.
产品详情
Target Species	Human
Product Form	Ig fraction - liquid
Preparation	Ig fraction prepared by chromatography
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Immunogen	Human fetal cord serum.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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数据
Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein (4520-5306) as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (MCA5862) as a detection reagent with human alpha fetoprotein (Bio-Rad Catalog 13752600) as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

技术参数

产品应用 ELISA

产品类型 Monoclonal Antibody

推荐用于 ELISA

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Bio-Rad 4520-5306 Alpha Fetoprotein,Mouse anti Human Alpha Fetoprotein

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