Sheep anti cytosine deaminase antibody bio-rad,Sheep anti cytosine deaminase antibody

2024-10-22

Sheep anti cytosine deaminase antibody

货号:2485-4906

规格:1 ml

价格:3120

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:Yeast

宿主:Sheep

抗体亚型:IgG3

荧光染料:Alexa Fluor 488

产品介绍
Sheep anti cytosine deaminase antibodyrecognizes cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognizes the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004).
产品详情
Target SpeciesYeast
Product FormPurified IgG - liquid
PreparationPurified Ig prepared by affinity chromatography on Protein G
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogePurified yeast cytosine deaminase.
Approx. Protein ConcentrationsIg concentration 5mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/5001/2500
Western Blotting1/1001/500
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Rabbit anti Sheep IgG (H/L):Biotin5184-2304F,E0.5mg
Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GAF,IF0.5mg
文献
1.Fuerer, C.et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.Gene Ther. 11: 142-512.Hammer, K.et al.(2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.Int J Cancer. 137 (4): 978-90.3.Amon, A.et al.(2008) Methods and Composition for Diagnosing and Treating CancerUS patent application US 11/767,2004.Pan, X.et al.(2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.Pancreas. 26: 274-8.5.Conrad, C.et al.(2005)Δ24-hyCD adenovirus suppresses glioma growthin vivoby combining oncolysis and chemosensitization.Cancer Gene Ther. 12: 284-94.6.Michnick, S.W.et al.(2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.Methods Enzymol. 470: 335-68.7.Komissarov, A.et al.(2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.Mol Biol Rep. 44 (4): 323-32.8.Hanauer, J.R.H.et al.(2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].

    技术参数

    产品应用 Elisa;WB

    产品类型 Polyclonal Antibody

    推荐用于 Elisa;WB

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