Bio-Rad 2330-4809 cortisol monoclonal ,Mouse anti cortisol monoclonal antibody

2024-10-22

Mouse anti cortisol monoclonal antibody

货号:2330-4809

规格:0.2 mg

价格:4573

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:cortisol monoclonal

物种:Broad

宿主:小鼠

抗体亚型:IgG3

克隆号:C13 (BGN/C13)

荧光染料:Purified

产品介绍
Mouse anti cortisol monoclonal antibody, clone C13recognizes cortisol, a glucocorticoid hormone synthesized from cholesterol by the adrenal cortex. It is released in response to pituitary adenocorticotrophic hormone (ACTH) which is, in turn, regulated by corticotrophin releasing factor (CRF). High levels of cortisol act to regulate the release of ACTH and CRF via negative feedback inhibition.The release of cortisol stimulates the conversion of proteins to carbohydrates, raises blood sugar levels and promotes glycogen storage in the liver.The following cross reactivities were observed:Cortisol 100%Prednisolone 30%Cortisone 25%Corticosterone 10%11-desoxycortisol 5%No reactivity was observed with 17-a-OH-progesterone.
产品详情

Target Species

Broad

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%Sodium Azide (NaN3)

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Immunogen

Cortisol conjugated to bovine serum albumin.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/50

1/1500

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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文献

1. Arya, S.K.et al.(2010) Antibody functionalized interdigitated micro-electrode (IDmicroE) based impedimetric cortisol biosensor.Analyst. 135 (8): 1941-6.2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA DetectionBiosensors Journal 1: H1106013. Arya, S.K.et al.(2010) Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.Biosens Bioelectron. 25: 2296-301.4. Arya, S.K.et al.(2011) Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor.Biosens Bioelectron. 28 (1): 166-73.5. Kaushik, A.et al.(2013) Mediator and label free estimation of stress biomarker using electrophoretically deposited Ag@AgO-polyaniline hybrid nanocomposite.Biosens Bioelectron. 50: 35-41.

技术参数

产品应用 ELISA

产品类型 Monoclonal Antibody

推荐用于 ELISA

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