Bio-Rad 2329-9907 Corticotrophin Releasing Facto,Mouse anti Human Corticotrophin Releasing Factor antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 2329-9907 Corticotrophin Releasing Facto,Mouse anti Human Corticotrophin Releasing Factor antibody

2024-10-22

Mouse anti Human Corticotrophin Releasing Factor antibody

货号：2329-9907

规格：0.2 mg

价格：3043

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原： Corticotrophin Releasing Facto

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：4H9

荧光染料：Purified

产品介绍
Mouse anti Human Corticotrophin Releasing Factor antibody, clone 4H9recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
产品详情
Target Species	Human
Product Form	Purified Ig - liquid
Preparation	Purified Ig prepared by affinity chromatography on Protein G
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations	1mg/ml
Immunogen	Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
Affinity	Averages 10(e)-9 M with native and modified CRF.
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Immunofluorescence	√
Immunohistology - Paraffin¹	√	1/100	1/7000
Immunohistology - Frozen	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献
1. Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants.Hybridoma. 13(1): 59-64.2. DeBellis, A.et al.(2012) Involvement of hypothalamus autoimmunity in patients with autoimmune hypopituitarism: role of antibodies to hypothalamic cells.J Clin Endocrinol Metab. 97 (10): 3684-90.

技术参数

产品应用 IHC(F);ELISA;IF;IHC(P)*

产品类型 Monoclonal Antibody

推荐用于 IHC(F);ELISA;IF;IHC(P)*

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Bio-Rad 2329-9907 Corticotrophin Releasing Facto,Mouse anti Human Corticotrophin Releasing Factor antibody

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