Bio-Rad 抗体,Native Mouse Collagen I/III

2024-10-22

Native Mouse Collagen I/III

货号:2150-2506

规格:10 mg

价格:2635

产品类型:蛋白

品牌:Bio-Rad

产品介绍
Murine collagen type I 45%Murine collagen type III 45%Murine collagen type IV 10%Murine collagen type V <1%Non-collagenous proteins <0.5%M[a1(I)1a2(I)2]. Native triple helix.
产品详情

Target Species

Mouse

Product Form

Purified protein from murine tail tendons - lyophilized

Preparation

Pepsin treatment, acetic acid extraction, serial salt precipitations

Buffer Solution

Essentially salt free

Preservative Stabilisers

None present

Reconstitution

Use 0.05-0.5 M acetic acid, pH 2.5 at 4ºC. Dissolved collagen retains immunologic properties of native collagen types I+III. Thermal denaturation converts dissolved collagen to gelatin.

Purity

Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement and ability to form microfibrils.

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.

Protein Molecular Weight

300 kDa
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

文献

1. Rhodes, R.K. & Miller, E.J. (1978) Physicochemical characterization and molecular organization of the collagen A and B chains.Biochemistry. 17 (17): 3442-8.

技术参数

产品应用 ELISA

产品类型 Purified Protein

推荐用于 ELISA

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