Bio-Rad ZAP 70 antibody 1065002S,Mouse anti Human ZAP 70:FITC

2024-10-22

Mouse anti Human ZAP 70:FITC

货号:1065002S

规格:25 µg

价格:2227

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:ZAP 70

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:SBZAP

荧光染料:FITC

产品介绍

Mouse anti Human ZAP 70 antibody, clone SBZAPrecognizes human ZAP-70, a 70kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.

产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

Immunogen

Synthetic peptide corresponding to amino acids 280-309 of human ZAP70 conjugated to keyhole limpet hemocyanin (KLH).

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Max Ex/Em

Fluorophore

Excitation Max (nm)

Emission Max (nm)

FITC

490

525

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

应用

Application Name

Verified

Min Dilution

Max Dilution

Flow Cytometry

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™(Product CodeBUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

相关产品

Description

Product Code

Applications

Pack Size

Human Seroblock

BUF070A

F

50 Test

Human Seroblock

BUF070B

F

200 Test

Leucoperm

BUF09

F

50 Tests

Leucoperm

BUF09B

F

250 Tests

Leucoperm

BUF09C

F

1000 Tests

文献

1.Roifman, C.M.et al.(1989) Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.J Exp Med. 170 (6): 2177-82.2.Arpaia, E.et al.(1994) Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase.Cell. 76 (5): 947-58.

技术参数

产品应用 F*

产品类型 Monoclonal Antibody

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