Bio-Rad 2150-0030,Native Human Collagen I-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 2150-0030,Native Human Collagen I

2024-10-22

Native Human Collagen I

货号：2150-0030

规格：0.1 mg

价格：3936

产品类型：蛋白

品牌：Bio-Rad

产品介绍
Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
产品详情
Target Species	Human
Product Form	Purified protein from human placenta - lyophilized
Preparation	Collagen from washed dissected tissue was dissolved in acetic acid after mild pepsin treatment. Collagen type I was then purified using differential salt precipitation.
Reconstitution	Use 0.05 - 0.5 M acetic acid, pH 2.5 at 4ºC.
Preservative Stabilisers	None present
Purity	>90%
Protein Molecular Weight	300 kDa
Regulatory	For research purposes only
Guarantee	Guaranteed until date of expiry. Please see product label.
Acknowledgements	The human source was tested and found negative for HBsAg ans HIV-1/2 antibodies.
存储条件
Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
数据
Sandwich ELISA analysis of human ferritin using Mouse anti Human collagen I/II/III antibody, clone NLI/22 (2150-2203) as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (131008) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4.

技术参数

产品应用 ELISA

产品类型 Purified Protein

推荐用于 ELISA

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Bio-Rad 2150-0030,Native Human Collagen I

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