Thermo Fisher DNA内切酶 快酶Esp3I FD0454,FastDigest Esp3I (IIs class) (BsmBI、BstGZ53I、Esp16I、Esp23I)/快酶Esp3I

2024-10-24

FastDigest Esp3I (IIs class) (BsmBI、BstGZ53I、Esp16I、Esp23I)/快酶Esp3I

货号: FD0454

规格:20 reactions

价格:926

产品类型:内切酶

品牌:Thermo Fisher

Thermo Scientific FastDigest Esp3I (IIs class) restriction enzyme recognizes CGTCTC(1/5)^ site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BsmBI, BstGZ53I, Esp16I, Esp23I.Thermo Scientific FastDigest Esp3I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymesfor a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such asKlenow Fragment,T4 DNA Ligase, alkaline phosphatases andT4 DNA Polymeraseall have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Note:FastDigest Esp3I is supplied in a storage buffer containing 7mM 2-mercaptoethanol.The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.Thermo Scientific FastDigest Esp3I(IIs 级)限制性内切酶可识别 CGTCTC(1/5)^ 位点,并使用通用型 FastDigest 缓冲液于 37°C 下在 5–15 分钟内的切割效果最佳。同裂酶:BsmBI、BstGZ53I、Esp16I、Esp23I。Thermo Scientific FastDigest Esp3I 是一系列高端快速限制性内切酶之一,这些酶在通用 FastDigest 缓冲液和 FastDigest 绿色反应缓冲液中均具有 100% 活性。通用缓冲液可在 5–15 分钟内对单股、双股或多股 DNA 进行快速酶切,无需更换缓冲液或后续 DNA 纯化步骤。有关该酶和其他 FastDigest 限制性内切酶的酶活性、热灭活和孵育时间的表格,请参见FastDigest 酶的反应条件。DNA 修饰酶(如Klenow 片段、T4 DNA 连接酶、碱性磷酸酶和T4 DNA 聚合酶)在 FastDigest 缓冲液中均具有 100% 活性。因此,在下游应用中使用的酶可直接添加至 FastDigest 反应混合物中。为了方便起见,FastDigest绿色缓冲液包括用于直接将酶切反应产品上样至凝胶的 1 种密度试剂和 2 种示踪染料。较短的孵育时间和较优的通用型 FastDigest 缓冲液组成消除了星活性效应。备注:FastDigest Esp3I 以储存缓冲液形式供货,含有7mM 2-巯基乙醇。FastDigest 绿色缓冲液在 DNA 酶切反应和下游应用中具有与无色 FastDigest 缓冲液一样的高性能。对于需要通过荧光激发进行产物分析的应用(例如在紫外光下测定浓度),则建议使用无色 FastDigest 缓冲液。对 dam 甲基化不敏感、对 dcm 甲基化不敏感、对 CpG 甲基化敏感。
优点:
- 100% activity of all FastDigest enzymes in the universal buffer- 100% buffer compatibility with downstream applications- Complete digestion in 5–15 minutes- Direct loading on gels- No star activity- 176 FastDigest enzymes available- 所有 FastDigest 酶在通用型缓冲液中的 100% 活性- 与下游应用的 100% 缓冲液兼容性- 5–15 分钟内即可完成酶切- 直接上样至凝胶- 无星号活性- 可提供 176 种 FastDigest 酶
数据:

技术参数

产品优点 1. 所有 FastDigest 酶在通用型缓冲液中的 100% 活性 2. 与下游应用的 100% 缓冲液兼容性 3. 5–15 分钟内即可完成酶切 4. 直接上样至凝胶 5. 无星号活性 6. 可提供 176 种 FastDigest 酶

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •单核苷酸多态性(SNP)

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