Bio-Rad 2150-0020 Collagen I,Rabbit anti Human Collagen I

2024-10-22

Rabbit anti Human Collagen I

货号:2150-0020

规格:50 µg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Collagen I

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human collagen I antibodyrecognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.
产品详情

Target Species

Human

Product Form

Purified IgG - lyophilized

Preparation

Purified IgG prepared by affinity chromatography on immobilized Collagen type I

Buffer Solution

0.1M Sodium Chloride0.01M Sodium Phosphate0.01M Sodium Borate

Preservative Stabilisers

1% Mannitol1% Dextran

Reconstitution

Reconstitute with 0.5ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.

Immunogen

Collagen type I from human placenta

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.
存储条件
Prior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/500

1/3000

Immunohistology - Paraffin1

1/101/50

Immunohistology - Frozen

1/101/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

1This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Description

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Pack Size

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数据

Published customer image:Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sectionsImage caption:Histological stains after marrow stimulation. Histological stains of cartilage repair tissue collected from 43-year-old patient 8.2 months after marrow stimulation surgery that required revision. Section outlined in blue in H&E stain is fibrocartilage, while the remaining tissue is primarily hyaline-like cartilage. Scale bars = 500 μm.
文献

1. Evans, M.J.et al.(2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.Am J Physiol Lung Cell Mol Physiol. 283 (6): L1263-70.2. Kim, H.J. & Im, G.I. (2010) The effects of ERK1/2 inhibitor on the chondrogenesis of bone marrow- and adipose tissue-derived multipotent mesenchymal stromal cells.Tissue Eng Part A. 16: 851-60.3. Lee, J.S. and Im, G.I. (2010) Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.Tissue Eng Part A. 16: 3569-77.4. Kim, Y.J.et al.(2008) PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs.Biochem Biophys Res Commun. 373: 104-85. Li Met al.(2013) Short periods of cyclic mechanical strain enhance triple-supplement directed osteogenesis and bone nodule formation by human embryonic stem cellsin vitro.Tissue Eng Part A. 19 (19-20): 2130-7.6. Hoffman, J.K.et al.(2015) Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.Case Rep Orthop. 2015: 617365.

技术参数

产品应用 IHC(F);ELISA;IHC(P)*

产品类型 Polyclonal Antibody

推荐用于 IHC(F);ELISA;IHC(P)*

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