IL-1 alpha Rat ELISA Kit ELISA试剂盒,IL-1 alpha Rat ELISA Kit/大鼠IL-1 α ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-1 alpha Rat ELISA Kit ELISA试剂盒,IL-1 alpha Rat ELISA Kit/大鼠IL-1 α ELISA试剂盒

2024-10-23

IL-1 alpha Rat ELISA Kit/大鼠IL-1 α ELISA试剂盒

货号：BMS627,BMS627TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	4.0 pg/mL
检测范围	15.6-1,000 pg/mL
样本类型/体积	血清 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	10%
批内变异系数（CV）	5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-1 alpha
Protein aliases	IL-1 alpha, Interleukin-1 alpha
物种（Tested）	大鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-1 alpha, Il1a
Gene ID	(Rat) 24493
Gene symbol	Il1a
UniProt ID	(Rat) P16598

The Rat Interlekin-1 alpha (Rt IL-1α) ELISA quantitates Rt IL-1α in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat IL-1α.大鼠白介素-1 α（大鼠IL-1α）ELISA定量了大鼠血清或细胞培养基中的大鼠IL-1α。该检测试剂盒将只识别天然和重组大鼠IL-1α。Principle of the method 方法原理The Rat IL-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠IL-1α固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.IL-1 α（白介素-1 α）是一种由单核细胞，巨噬细胞和树突状细胞表达的促炎细胞因子。 IL-1 α由IL1A基因编码，并通过两个受体IL-1RI和IL-1RII发出信号，这两个受体均与IL-1β共享。 IL1A位于13号染色体2的q臂上。IL-1α通过触发靶细胞中其他促炎细胞因子的产生并启动急性期反应，在先天宿主防御中发挥重要作用。 IL-1受体拮抗剂（IL-1RA）可以调节IL-1α的活性，IL-1RA是许多细胞类型产生的一种蛋白质，通过竞争性抑制作用阻断受体结合。 IL-1α是参与多种免疫反应，炎症过程和造血作用的多效细胞因子。此外，IL-1α由单核细胞和巨噬细胞产生为前蛋白，其被蛋白水解加工并响应细胞损伤而释放并诱导凋亡。 IL1A基因和其他8个白介素1家族基因在2号染色体上形成一个细胞因子基因簇。IL1α调节NF-κB和丝裂原激活的蛋白激酶的活性。 IL-1α可以刺激IL6和环氧合酶2（PTGS2 / COX-2）的表达，并增强一氧化氮（NO）的产生。高水平的IL-1α与几种慢性炎症疾病有关，包括类风湿关节炎，牛皮癣和多发性硬化症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-1 alpha Rat ELISA KitRepresentative standard curve for rat IL-1 alpha ELISA

技术参数

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IL-1 alpha Rat ELISA Kit ELISA试剂盒,IL-1 alpha Rat ELISA Kit/大鼠IL-1 α ELISA试剂盒

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