产品介绍 | | |
| Mouse anti Human Apolipoprotein A1, clone G2recognizes Apolipoprotein A-1 (also known as Apo-A1) , a lipid-binding protein which enables the transport of dietary lipids for storage, metabolism and secretion. Apo-A1 plays an important part in the removal of cholesterol from cells.Mouse anti Human Apolipoprotein A1, clone G2 reacts with both free human Apo-A1 and High Density Lipoprotein (HDL) bearing Apo-A1, but does not cross-react with ApoE, ApoB or Albumin. |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Reconstitution | Reconstitute with 1.0ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from ascites |
Buffer Solution | 0.01M Sodium Phosphate0.01M Sodium Borate0.15M Sodium Chloride |
Preservative Stabilisers | 1% Dextran1% Mannitol |
Immunogen | Native Apolipoprotein-A1 from human plasma |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/5000 |
Immunohistology- Frozen | √ | | 1/40 |
Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.- ELISA
- This antibody is suitable for coating microtitre plates in a sandwich ELISA using catalogue number0650-0190for detection.
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文献 |
| 1.Derbali, H.et al.(2010) Increased biglycan in aortic valve stenosis leads to the overexpression of phospholipid transfer protein via Toll-like receptor 2.Am J Pathol. 176: 2638-45.2.Mogilenko, D.A.et al.(2012) Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages.FASEB J. 26: 2019-30.3.Shavva, V.S.et al.(2016) PPARγ Represses Apolipoprotein A-I Gene but Impedes TNFα-Mediated ApoA-I Downregulation in HepG2 Cells.J Cell Biochem. 117 (9): 2010-22.4.Pingitore, P.et al.(2016) Identification and characterization of two novel mutations in the LPL gene causing type I hyperlipoproteinemia.J Clin Lipidol. 10 (4): 816-23.5.Berge, K.E.et al.(2014) Type 1 hyperlipoproteinemia due to a novel deletion of exons 3 and 4 in the GPIHBP1 gene.Atherosclerosis. 234 (1): 30-3.6.Botta, M.et al.(2019) Deciphering the role of V200A and N291S mutations leading to LPL deficiency.Atherosclerosis. 282: 45-51. |
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