Mouse anti Human IgE antibody, clone E411 (5H2) bio-rad,Mouse anti Human IgE antibody, clone E411 (5H2)

2024-10-22

Mouse anti Human IgE antibody, clone E411 (5H2)

货号:0100-0413,0100-0413GA

规格:1 mg,0.2 mg

价格:5100,3043

产品类型:流式抗体

品牌:Bio-Rad

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:E411 (5H2)

荧光染料:Purified

产品介绍
Mouse anti Human IgE antibody, clone E411 (5H2)recognizes human IgE heavy chain, a 428 amino acid protein bearing four Ig-like domains. Mouse anti Human IgE antibody, clone E411 binds to an epitope expressed on Cε3 domain.Mouse anti Human IgE antibody, clone E411 has been successfully used as a capture reagent in the develpment of a sensitive Sandwich ELISA in combination with biotinylated Goat anti Human IgE (STAR147B) as a detection reagent. It hs also been used in the development of a bead based microfluidic assay for the measurement of patient IgE levels in combination with Mouse anti Human IgE antibody, clone E454 (0100-0414) indicating potential for the study and monitoring of IgE levels in allergic events (Proczeket al.2012).
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
Preparation0100-0413: Purified IgG prepared by affinity chromatography on Protein G from ascites0100-0413GA: Purified IgG prepared by affinity chromatography on Protein G
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers<0.1% Sodium Azide (NaN3)
ImmunogeImmunoglobulin E from human serum
Approx. Protein Concentrations1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF0.5mg
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE,WB0.5mg
同型对照
DescriptionProduct CodeApplicationsPack Size
Mouse IgG2a Negative ControlMCA929F100Tests
文献
1De Paula, M.L.et al.(2014) The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF-1 signaling in rat and human oligodendrocyte progenitors.Glia. 62 (1): 64-77.2。Preisner, A.et al.(2015) Non-steroidal anti-inflammatory drug indometacin enhances endogenous remyelination.Acta Neuropathol. 130 (2): 247-61.3.Kuroda, M.et al.(2017) Peripherally derived FGF21 promotes remyelination in the central nervous system.J Clin Invest. 127 (9): 3496-509.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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