Bio-Rad 0178-0204 ACTH,Rabbit anti Human ACTH

2024-10-23

Rabbit anti Human ACTH

货号:0178-0204

规格:0.5 ml

价格:2933

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:ACTH

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human ACTH antibodyrecognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5 kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol.Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisioet al.2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP).
产品详情

Target Species

Human

Product Form

Purified Ig - liquid

Preparation

Purified Ig prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%Sodium Azide (NaN3)

Approx. Protein Concentrations

5.0 mg/ml

Immunogen

Synthetic peptide corresponding to the amino acids 1-24 (SYSMEHFRWGKPVGKKRRPVKVYP) of ACTH conjugated to Keyhole limpet Haemocyanin.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/500

1/1000

Immunofluorescence

Immunohistology - Paraffin1/1001/200

Immunohistology - Frozen

1/1001/200
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.Histology Positive Control TissueACTH-containing cells of the anterior pituitary gland.
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文献

1. Osamura, R.Y.et al.(1980) Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.Am J Pathol. 99 (1): 105-24.2. Fiordelisio, T.et al.(2006) Cells of proopiomelanocortin lineage from the rodent anterior pituitary lack sexually dimorphic expression of neurofilaments.Neuroendocrinology. 83:360-70.3. Farris, G.M.et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.Toxicol Sci. 97: 548-61.

技术参数

产品应用 IHC(F);ELISA;IF;IHC(P)

产品类型 Polyclonal Antibody

推荐用于 IHC(F);ELISA;IF;IHC(P)

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