CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), eBioscience

货号：14-0211-81,14-0211-37,14-0211-95

规格：50 μg,2 mg,10 mg

市场价格：822,11562,28906

产品类型：流式抗体

品牌：eBioscience

抗原：CD21/CD35

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：eBio8D9 (8D9)

荧光染料：其它

类型:	流式抗体	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)； Assay-Dependent（WB）; Assay-Dependent(FN)

产品详细信息Description:

The monoclonal antibody eBio8D9 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which produce cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is some evidence which demonstrates their expression on macrophages. CD21 is a receptor for the complement component C3d and Epstein-Barr virus (EBV). In association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2-deficient mice display impaired inflammatory and humoral immune responses in vivo.The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other, suggesting that they bind to different epitopes.Applications Reported: This eBio8D9 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The eBio8D9 antibody can also be used in functional assays to crosslink CD21/CD35 both to itself and to sIgM.Applications Tested: This eBio8D9 (8D9) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD21/CD35 Antibody (14-0211-81) in FlowStaining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD21/CD35 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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参考文献：

1.The Journal of experimental medicineFate mapping reveals origin and dynamics of lymph node follicular dendritic cells."14-0211 was used in Immunofluorescence to investigate the ontogeny and dynamics of lymph node follicular dendritic cells in situ."2.Science advancesDiscrete roles and bifurcation of PTEN signaling and mTORC1-mediated anabolic metabolism underlie IL-7-driven B lymphopoiesis."14-0211 was used in Flow cytometry/Cell sorting to determine the mechanism by which interleukin-7 in early B lymphopoeisis and the role of Stat5-dependent and independent pathways in this process."3.Scientific reportsMicroRNA-449a deficiency promotes colon carcinogenesis."14-0211 was used in Flow cytometry/Cell sorting to suggest that miR-449a acted as a key regulator of colon tumorigenesis by controlling the proliferation of intestinal epithelial cells."4.PloS oneCRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development."14-0211 was used in Flow cytometry/Cell sorting to elucidate the contribution of the SLAM receptors in immunodysregulation observed in X-linked lymphoproliferative syndrome Type 1."

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