| 产品介绍 | | |
| Mouse anti Human CD21 antibody, clone LB21recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
Sheep | √ |
Mink | √ |
Bovine | √ |
Goat | √ |
Cat | √ |
N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Reconstitution | Pack Size: 100 Tests |
Reconstitute with 1 ml distilled water |
Pack Size: 25 Tests |
Reconstitute in 0.25 ml disilled water |
Preparation | Purified IgG prepared by ion exchange chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
Immunogen | Human IM9 cell line. |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood |
| 同型对照 |
Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
| 相关产品 |
Description | Product Code | Applications | Pack Size |
Human Seroblock | BUF070A | F | 50 Test |
Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647) and FITC conjugated Mouse anti Human CD21 (MCA1195F). All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
| 文献 |
| 1. Furukawa, Y.et al.(2000) Frequent reversible membrane damage in peripheral blood B cells in human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP).Clin Exp Immunol. 120 (2): 307-16.2. Griebel, P.J.et al.(2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.Vet Immunol Immunopathol. 119: 115-22.3. Aasted, B.et al.(2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.Vet Immunol Immunopathol. 119: 27-37.4. Sopp, P.et al.(2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.Vet Immunol Immunopathol. 119: 106-14.5. Davis, W.C.et al.(2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.Vet Immunol Immunopathol. 119: 123-30.6. Aichem, A.et al.(2006) Redox regulation of CD21 shedding involves signaling via PKC and indicates the formation of a juxtamembrane stalk.J Cell Sci. 119: 2892-902.7. Angel, C.E.et al.(2009) Distinctive localization of antigen-presenting cells in human lymph nodes.Blood. 113: 1257-67.8. Sengstake, S.et al.(2006) CD21 and CD62L shedding are both inducible via P2X7Rs.Int Immunol. 18: 1171-8.9. Clutterbuck, E.A.et al.(2012) Pneumococcal conjugate and plain polysaccharide vaccines have divergent effects on antigen-specific B cells.J Infect Dis. 205: 1408-16.10. Meister, R.K.et al.(2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.Vet Immunol Immunopathol. 119: 38-46.11. Damgaard, B.M.et al.(2012) The effects of feed restriction on physical activity, body weight, physiology, haematology and immunology in female mink.Res Vet Sci. 93: 936-42.12. Stachowiak, B. & Weingartl, H.M. (2012) Nipah virus infects specific subsets of porcine peripheral blood mononuclear cells.PLoS One. 7 (1): e30855. |
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