| 产品介绍 | | |
| Mouse anti Human CD21 antibody, clone LB21recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
Sheep | √ |
Mink | √ |
Bovine | √ |
Goat | √ |
Cat | √ |
N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by ion exchange chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
Immunogen | Human IM9 cell line. |
| Approx. Protein Concentrations | IgG concentration 25 ug/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | | Neat |
Immunohistology - Frozen1 | √ | | |
Immunohistology - Paraffin | √ | 1/10 | 1/100 |
Immunoprecipitation | | | |
| 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.Histology Positive Control TissueLymph node |
| 同型对照 |
Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
| 相关产品 |
Description | Product Code | Applications | Pack Size |
Human Seroblock | BUF070A | F | 50 Test |
Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MCA551), red in A and Mouse anti Human CD21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.Immunoperoxidase staining of human spleen cryosection using Mouse anti Human CD21 antibody (MCA1195). Low power. |
| 文献 |
| 1. Furukawa, Y.et al.(2000) Frequent reversible membrane damage in peripheral blood B cells in human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP).Clin Exp Immunol. 120 (2): 307-16.2. Griebel, P.J.et al.(2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.Vet Immunol Immunopathol. 119: 115-22.3. Aasted, B.et al.(2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.Vet Immunol Immunopathol. 119: 27-37.4. Sopp, P.et al.(2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.Vet Immunol Immunopathol. 119: 106-14.5. Davis, W.C.et al.(2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.Vet Immunol Immunopathol. 119: 123-30.6. Aichem, A.et al.(2006) Redox regulation of CD21 shedding involves signaling via PKC and indicates the formation of a juxtamembrane stalk.J Cell Sci. 119: 2892-902.7. Angel, C.E.et al.(2009) Distinctive localization of antigen-presenting cells in human lymph nodes.Blood. 113: 1257-67.8. Sengstake, S.et al.(2006) CD21 and CD62L shedding are both inducible via P2X7Rs.Int Immunol. 18: 1171-8.9. Clutterbuck, E.A.et al.(2012) Pneumococcal conjugate and plain polysaccharide vaccines have divergent effects on antigen-specific B cells.J Infect Dis. 205: 1408-16.10. Meister, R.K.et al.(2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.Vet Immunol Immunopathol. 119: 38-46.11. Damgaard, B.M.et al.(2012) The effects of feed restriction on physical activity, body weight, physiology, haematology and immunology in female mink.Res Vet Sci. 93: 936-42.12. Stachowiak, B. & Weingartl, H.M. (2012) Nipah virus infects specific subsets of porcine peripheral blood mononuclear cells.PLoS One. 7 (1): e30855. |
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