Bio-Rad MCA1210P647 小鼠IgG2a同型,Mouse IgG2a Negative Control:PE-Alexa Fluor 647-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1210P647 小鼠IgG2a同型,Mouse IgG2a Negative Control:PE-Alexa Fluor 647

2024-10-23

Mouse IgG2a Negative Control:PE-Alexa Fluor 647

货号：MCA1210P647

规格：100 Tests/1ml

价格：2635

产品类型：小鼠同型对照

品牌：Bio-Rad

物种：其它

宿主：小鼠

抗体亚型：IgG2a

荧光染料：PE-Alexa Fluor 647

产品介绍
Mouse IgG2a Negative Controlis suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.
产品详情
Target Species	Negative Control
Product Form	Purified IgG conjugated to R. Phycoerythrin Alexa Fluor®750 - lyophilized
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1%Bovine Serum Albumin 5% Sucrose
Immunogen	Human T lymphocytes.
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE-Alexa Fluor®647 488nm laser	496	667
	RPE-Alexa Fluor®647 561nm laser	546	667
Regulatory	For research purposes only
Guarantee	6 monthsfrom date of despatch
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		*
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells in 100ul
数据
Figure A. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse IgG2a isotype control (MCA1210PE). Figure B. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse anti rat CD2 (MCA154PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
文献
1. Fijak, M.et al.(2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.Am J Reprod Immunol. 74 (1): 12-25.

技术参数

产品应用 Flow

产品类型 Negative/Isotype Control

推荐用于 Flow

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Bio-Rad MCA1210P647 小鼠IgG2a同型,Mouse IgG2a Negative Control:PE-Alexa Fluor 647

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