Bio-Rad 小鼠IgG2a同型 MCA1210F,Mouse IgG2a Negative Control:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 小鼠IgG2a同型 MCA1210F,Mouse IgG2a Negative Control:FITC

2024-10-23

Mouse IgG2a Negative Control:FITC

货号：MCA1210F

规格：0.1 mg

价格：2695

产品类型：小鼠同型对照

品牌：Bio-Rad

物种：其它

宿主：小鼠

抗体亚型：IgG2a

荧光染料：FITC

产品介绍
Mouse IgG2a Negative Controlis suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.
产品详情
Target Species	Negative Control
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1%Bovine Serum Albumin
Immunogen	Human T lymphocytes.
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		*
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells in 100ul
数据
Figure A. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse IgG2a isotype control (MCA1210F). Figure B. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse anti rat CD11b (MCA619F). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.
文献
1. Fijak, M.et al.(2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.Am J Reprod Immunol. 74 (1): 12-25.

技术参数

产品应用 Flow

产品类型 Negative/Isotype Control

推荐用于 Flow

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Bio-Rad 小鼠IgG2a同型 MCA1210F,Mouse IgG2a Negative Control:FITC

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