| 产品介绍 | | |
| Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a 55 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.Mouse anti human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (Piatier-Tonneau et al, 1997). Bio-Rad recommend the use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) for this purpose. |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | Human PHA blasts |
Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE-Alexa Fluor®647 488nm laser | 496 | 667 |
| RPE-Alexa Fluor®647 561nm laser | 546 | 667 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents. |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood.Tandem conjugates of RPE-Alexa Fluor® 647 display fluorescence properties that are virtually identical to those of RPE-Cy5 tandem conjugates. |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Figure A. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). Figure B. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647) and StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
| 文献 |
| 1.monocytes.J Immunol. 167 (8): 4593-9.2.Voehringer, D.et al.(2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1).Blood. 100 (10): 3698-702.3.Piatier-Tonneau, D. (1997) CD4 workshop panel report.In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.4.Pentón-Rol, G.et al.(2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.Int Immunopharmacol. 11 (1): 29-38.5.Wright, G.J.et al.(2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.Immunology. 102 (2): 173-9.6.Zhang, Y.et al.(2013) Acceleratedin vivo.proliferation of memory phenotype CD4+T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.PLoS Pathog. 9 (4): e1003310.7.Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088. |
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