VGLL4 Recombinant Rabbit Monoclonal Antibody (16H12L24)

货号：703012

规格：100 µg

价格：4643

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:100(ICC/IF)

产品详细信息This antibody is predicted to react with Monkey, Horse, Cat.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Transcription cofactor vestigial-like protein 4 (VGLL4) acts as a specific coactivator for the mammalian TEFs

数据

VGLL4 Antibody (703012) in ICC/IFKnockdown of VGLL4 was achieved by transfecting HCTt116 cells with specific siRNA (Silencer® select Product # s229766, s229764). Immunofluorescence analysis was performed on HCT116 cells (untransfected, panel a-d), transfected with VGLL4 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703012, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to VGLL4 (green). The images were captured at 60X magnification.

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