H3K9acK14ac Polyclonal Antibody

货号：49-1010

规格：44 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人/小鼠/非人灵长类/猪/恒河猴

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:500 (WB)；1:200-1:250(ICC/IF)；1:150,000(ELISA)；2 µg (ChIP)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据

H3K9acK14ac Antibody (49-1010) in ICC/IFImmunofluorescence analysis of Acetyl-Histone H3 (Lys9, Lys14) was performed using 70% confluent log phase HeLa cells treated with Trichostatin A (0.4uM, 18hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys9, Lys14) Polyclonal Antibody (Product # 49-1010) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing increase in nuclear localization. Panel e represents control cells showing very weak nuclear expression . Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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参考文献：

1.OncotargetCross-species genomic and epigenomic landscape of retinoblastoma."49-1010 was used in ChIP assay to analyze the cross-species genomic and epigenomic pattern of retinoblastoma"

技术参数

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